One, in a skilled nursing facility, led to rapid transmission and significant mortality in this vulnerable population but little broader spread, while other To achieve these aims, whole genome We report a genomic surveillance of SARS-CoV-2 lineages circulating in Paran, southern Brazil, from March 2020 to April 2021. Forster P, Forster L, Renfrew C, Forster M. Phylogenetic network analysis of SARS-CoV-2 genomes. 2020 Apr 16 [Epub]. We have performed a comparative phylogenetic analysis of the sequences, in order Page 17 Share Cite. The severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) viral genome is an RNA virus consisting of approximately 30,000 bases. Of the countries that have contributed SARS-CoV-2 The reported genomes belong to the S The phylogenetic network methods used by researchers allowing the visualization of hundreds of evolutionary trees simultaneously in one simple graph were pioneered in New Zealand in 1979, then developed by German mathematicians in the 1990s. Our analysis, based on 333 genomes, revealed that the first variants detected in the state of Paran in March 2020 were the B.1.1.33 and B.1.1.28 variants. However, in South America, this variant’s arrival and genomic diversity are scarcely Here, we built a phylogenetic tree with globally obtained 15,277 severe acute respi . Illumina sequence analysis. All 42 genomes were selected for phylogenetic analysis of SARS-CoV-2 from imported cases in Nanjing . Given the recent emergence of the Omicron (B.1.1.529) variant, this tool has provided data about this lineage’s genomic and epidemiological characteristics. Lessler J, Reich NG, Brookmeyer R, Perl TM, Nelson KE, Cummings DA: Incubation periods of acute respiratory viral infections: a systematic review. Key features of microbial genomes, focusing on SARS-CoV-2. The data revealed two superspreading events. Phylogenetic network analysis of SARS-CoV-2 genomes C19 receptor stucture and functional biology - Nature March2020 A Trial of LopinavirRitonavir in Adults Hospitalized with Severe Covid-19 Phan T. Genetic diversity and evooution of SARS-CoV-2. Eight-four different lineages have been identified from these countries with 86% genomes belonging to the B.1 sublineage and its descendants. Genetic recombination is a major evolutionary mechanism among RNA viruses, and it is common in coronaviruses, including those infecting humans. Here, we built a phylogenetic tree with globally obtained 15,277 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes. Describes the detection and genome sequencing of SARS-CoV-2 variants to prioritize COVID-19 investigation and contact tracing activities. a GISAID currently has thousands of SARS-CoV-2 genomic sequences banked. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. This pilot study aimed to describe the genetic variation and molecular epidemiology of SARS-CoV-2 in Palestine in fall 2020. 10. Phylogenetic Analysis and Structural Modeling of SARS-CoV-2 Spike Protein Reveals an Evolutionary Distinct and Proteolytically Sensitive Activation Loop. In this paper, we present a pipeline for subtyping SARS-CoV-2 viral genomes based on short sets of highly informative nucleotide sites (ISMs). Genomic surveillance of SARS-CoV-2 is one of the tools that provide genomic information on circulating variants. Method Genetic variation in 137 SARS-CoV-2 genomes The A and C types are found in significant proportions outside East Asia, Proceedings of the National Academy of Sciences , 2020; 202004999 DOI: 10.1073/pnas.2004999117 Cite This Page : We all get that the closest known ancestor to SARS-CoV-2 is that bat virus. Bat virus MG996532 is written in red, MG772933 and MG772934 are in blue. A comprehensive genomic characterization of the virus isolates in Turkey is yet to be carried out. Phylogenetic analysis identified the presence of all the five reported clades 19A, 19B, 20A, 20B and 20C in the population. e phylogenetic network analysis of SARS-CoV-2 undertaken in [24] belongs to the data-display category. Node A is the root cluster obtained with the bat ( Rhinolophus affinis) coronavirus isolate BatCoVRaTG13 from Clades of SARS-CoV-2 are in bold in all source phylogenetic ML trees. Grimm G, Morrison D. Harvest and phylogenetic network analysis of SARS virus genomes (CoV-1 and CoV-2). Forster, P et al. The current pandemic caused by the novel human coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously known as HCoV-19 first) emerged in Wuhan, China, in 2019 [].Since then, several studies have been developed to assess the stability of SARS-CoV-2 on different surfaces to stablish the real risk of virus spread through fomites Colloquially known as simply the coronavirus, it was previously referred to by its provisional name, 2019 novel coronavirus (2019-nCoV), and has also been called human that RaTG13 is not the direct progenitor of SARS-CoV-2 [18, 19]. Our SimPlot analysis (Fig. Viral phylogeny is based on the consensus sequence assembled of each sample, and the branching indicates the evolutionary differences in the consensus sequence between samples. USA 117 (2020) Akhter et al., Applying Shannons information theory to bacterial and phage genomes and metagenomes, Sci. (A) The Pango lineages of VOC Delta. Framework for identifying population-level SARS-CoV-2 strains and lower frequency quasispecies through pangenome analysis. The virus first emerged in Wuhan, China in December 2019, and subsequently spreaded around the world. Method Genetic variation in 137 SARS-CoV-2 genomes During the pandemic, whole genome sequencing has played an important role in understanding the evolution and genomic diversity of SARS-CoV-2. Figure. Nature. 2009, 7:439-50. The data revealed two superspreading events. Although an unprecedented number of SARS-CoV-2 full genomes have been There are too many rapid mutations to neatly trace a SARS-CoV-2 family tree. The coronavirus disease 19 (COVID-19) is a highly transmittable and pathogenic viral infection caused by a novel evolutionarily divergent RNA virus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The lineages are marked with different colors. The research revealed three distinct 'variants' of SARS-CoV-2, consisting of clusters of closely related lineages, which they label A, B and C. Additional samples, not reported in this study, were included on Illumina NextSeq runs. (B) The countries distribution of VOC Delta. Since its emergence in China, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide including Pakistan. Phylogenetic network of 160 SARS-CoV-2 genomes. Proceedings of the National Academy of Science USA. (2020) Phylogenetic network analysis of SARS-CoV-2 genomes. By analysing the first 160 complete virus genomes to be sequenced from human patients, the scientists have mapped some of the original spread of the new coronavirus through its mutations, which creates different viral lineages. Module 3.3. The raw reads were demultiplexed using bcl2fastq (v2.20) (Illumina Inc.) to produce 311 FASTQ files for the run with the routine samples (112 SARS-CoV-2 samples and 3 negative controls) and the run with the rapid response samples (247 SARS-CoV 2020 May 1; 432(10): 33093325. Proc Natl Acad Sci U S A. Here, we characterized the SARS-CoV-2 genomes isolated from five travelers who returned to Taiwan COVID-19 caused by SARS-CoV-2 virus emerged as a global pandemic worldwide. To investigate the introduction, spread, and epidemiology of COVID-19 in the Boston area, we sequenced and analyzed 772 complete SARS-CoV-2 genomes from the region, including nearly all confirmed cases within the first Phylogenetic analysis of 150 severe acute respiratory syndrome coronavirus 2 representative genome sequences from lineage B1, including genomes collected in Italy (blue) and sequences identified for this study at the National Institute for Infectious Diseases (red). Evolutionary history, potential intermediate animal host, and crossspecies analyses of SARSCoV2. Suggested Citation:"1 Introduction." Rooting a phylogenetic tree to something so distant almost makes it noise in determining the evolution of the current virus. characterisation of a novel SARS-CoV-2. This makes character-based phylogenetic networks the method of choice for Network, 26 Feb. 2020. In a phylogenetic network analysis of 160 complete human severe acute respiratory Renfrew C, Forster M. Phylogenetic network analysis of SARS-CoV-2 genomes. All viruses, including SARS-CoV-2, the virus that causes COVID-19, change over time. We identified the subtypes 9. To investigate the evolutionary and epidemiological dynamics of the current COVID-19 outbreak, a total of 112 genomes of SARS-CoV-2 strains sampled from China and 12 other countries with sampling dates between 24 December 2019 and 9 February 2020 were analyzed. Phylogenetic network analysis of SARS-CoV-2 genomes C19 receptor stucture and functional biology - Nature March2020 A Trial of LopinavirRitonavir in Adults Hospitalized with Severe Covid-19 Information on viral genomics is crucial for understanding global dispersion and for providing insight into viral pathogenicity and transmission. The four positively-tested RNA samples were further analysed at the IMB. Researchers are also looking at the SARS-CoV-2 genome for clues about its true origin: the animal that infected the first person. 1 Forster et al. The variants B.1.1.28 and B.1.1.33 were predominant throughout 2020 until the In this article, however, we focus on rooted phylogenetic networks, also known as explicit or evolutionary phylogenetic networks. Real-time phylogenetics with UShER. Even so, it is still quite stubby compared to the genomes of people, plants, and even bacteria. More information: Peter Forster et al, Phylogenetic network analysis of SARS-CoV-2 genomes, Proceedings of the National Academy of Sciences (2020). Module 1.3. A few SARS-CoV-2 recombinants have been reported to date whose genome harbored combinations of mutations from different mutants or variants, but only a single patient’s sample was analyzed, and the virus was not isolated. To assist in tracing infection pathways and design preventive strategies, a deep understanding of the viral Contorting its RNA into three-dimensional shapes gives SARS-CoV-2 another set of tools with which to compensate for a limited number of genes. The phylogenetic network analysis of SARS-CoV-2 undertaken in belongs to the data-display category. 2020, 117:9241-3. But it is still a thousand nucleotide differences away from the human SARS-CoV-2 causing a pandemic at the moment. To date, 23 countries from the WHO Africa region have deposited a total of 3995 SARS-CoV-2 sequences to publicly available databases, with the majority of the genomes being from South Africa (56%). It also offers classification or typing of the queried genome using EzBioClouds SNP based classification scheme of SARS-CoV-2 variants, including an evolutionary analysis of the detected SARS-CoV-2 type along with other types observed among publicly available SARS-CoV-2 genomes. In clus-ter-III, a star pattern was formed around the Wuhan (A) The evolutionary history was inferred using the Maximum Parsimony (MP) method. Figure 2. Proceedings of the National Academy of Sciences 117(17):92419243. Furthermore, the SARS-CoV-2 virus has geographically diverse strains that seemingly vary in severity, mortality rate, and treatment options that were characterized using phylogenetic network analysis of 160 SARS-CoV-2 genomes . Gene Reports. This is a phylogenetic network of SARS-CoV-2 genomes sampled from across the world. It was reproduced in the mainstream press, but the method used in the study had a number of shortcomings. Background There is a global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Background SARS-CoV-2 began spreading in December 2019 and has since become a pandemic that has impacted many aspects of human society. Phylogenetic analysis of 48 SARS-Cov-2 isolates from Western Serbia (c) in comparison to Wuhan complete genome sequence (NCBI accession number NC_045512.2) and 31 most similar SARS-Cov-2 sequences obtained from other world regions through BLAST search. The genome of the Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2), the pathogen that causes coronavirus disease 2019 (COVID-19), has been sequenced at an unprecedented scale leading to a tremendous amount of viral genome sequencing data. Natl. The viral lineage So far, bats appear to be the most likely suspect. Genomic analyses revealed that this Phylogenetic network analysis of SARS-CoV-2 genomes. Sequencing of viral genomes can help to answerand in some cases, may provide the only way to answerquestions that are Phylogenetic network analysis of SARS-CoV-2 genomes. Proc Natl Acad Sci USA 117: 92419243, 2020. doi: 10.1073/pnas.2004999117. Forster, P et al. As of May 2020, Turkey is among the top ten countries with the most cases. Nat Rev Microbiol. We report a genomic surveillance of SARS-CoV-2 lineages circulating in Paran, southern Brazil, from March 2020 to April 2021. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. The team used data from virus genomes sampled from across the world between 24 December 2019 and 4 March 2020. Rooting a phylogenetic tree to something so distant almost makes it noise in determining the evolution of the current virus. Proceedings of the National Academy of Science. Figure 1. DOI: 10.1073/pnas.2004999117 As of May 2020, Turkey is among the top ten countries with the most cases. Analysis of 772 complete severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes from early in the Boston-area epidemic revealed numerous introductions of the virus, a small number of which led to most cases. Given the recent emergence of the Omicron (B.1.1.529) variant, this tool has provided data about this lineage’s genomic and epidemiological characteristics. Analysis of 772 complete SARS-CoV-2 genomes from early in the Boston area epidemic revealed numerous introductions of the virus, a small number of which led to most cases. SARS-CoV-2 Phylogenetic Analysis, Italy. Phylogenetic Analysis of the SARS-CoV-2 Genomes. (2020) Phylogenetic network analysis of SARS-CoV-2 genomes. Module 2.7 an interactive tool for transmission network analysis. 10.1073/pnas.2004999117; Perlman S, Netland J: Coronaviruses post-SARS: update on replication and pathogenesis. 2 The mutation initially appeared to arise independently and simultaneously sweep across multiple geographic regions. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. (2013) Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the strain of coronavirus that causes coronavirus disease 2019 (COVID-19), the respiratory illness responsible for the COVID-19 pandemic. The A and C types are A comprehensive genomic characterization of the virus isolates in Turkey is yet to be carried out. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. Haplotype network and phylogenetic analysis of SARS-CoV-2 sequences. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. (2016) SARS-like WIV1-CoV poised for human emergence. Also, the two genomes show some divergence in the receptor-binding domain (RBD) of the spike protein [23]. By analysing 53 SARS-CoV-2 whole genome sequences collected up to February 3, 2020, we find a strong association between the On April 28, an article was published in the journal PNAS on phylogenetic network analysis of SARS-CoV-2 genomes. An initial genomic sequence analysis found that the reemergence of COVID-19 in New Zealand was caused by a SARS-CoV-2 from the (now ancestral) lineage B.1.1.1 of the pangolin nomenclature . 1a) conducted with 25 CoV genomes (see the Methods section for a detailed data description) shows that the Wuhan SARS-CoV-2 and RaTG13 genomes share 96.14% of whole-genome identity, while the Wuhan SARS-CoV-2 and GD Pangolin genomes are 90.34% identical. 2020 Apr 8 [Epub]. COVID-19 has effectively spread worldwide. 2020;117:92413. However, some changes may affect the viruss properties, such as how easily it spreads, the associated disease severity, or the performance of vaccines, therapeutic medicines, diagnostic tools, or other public health and COVID-19 has effectively spread worldwide. Genetic diversity of SARS-CoV-2 (formerly 2019-nCoV), the virus which causes COVID-19, provides information about epidemic origins and the rate of epidemic growth. The mutation rate estimated from early cases of SARS-CoV-2 was of 6.54 X 10-4 per site per year. (2020) Phylogenetic network analysis of SARS-CoV-2 genomes. For two of the four samples, having the highest virus loads (MHT_1/MHT_2; Table Table1), 1), whole genome sequencing of SARS-CoV-2 according to the nCoV-2019 sequencing protocol [] on a GridION instrument was successful.Briefly, after passing quality control, demultiplexing, adapter trimming Acad. The reported genomes belong to the S SARS-CoV-2, severe respiratory syndrome coronavirus-2, is an RNA virus that emerged from China sweeping the globe in the form of a pandemic that became an international public health concern. In this study, we used ARTIC primers based amplicon sequencing to profile 225 SARS-CoV-2 genomes from India. As part of testing efforts, whole genome sequencing of human isolates has resulted in over 1,600 complete genomes publicly available from GenBank. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. ian SARS-CoV-2 genomes, 32 retrieved genomes, Wuhan reference strain, and an out-group genome of the bat haplotypes. Journal of Medical Virology, 2020. The variants B.1.1.28 and B.1.1.33 were predominant throughout 2020 until the e haplotype network analysis supported the phylogenetic tree in showing three clusters. The VOC Delta were compared with GISAID-available SARS-CoV-2 genomes (n = 1,503, updated on October 09, 2021). 10.1038/nrmicro2147 Our analysis, based on 333 genomes, revealed that the first variants detected in the state of Paran in March 2020 were the B.1.1.33 and B.1.1.28 variants. These genomes are closely related and under evolutionary selection in their human hosts, sometimes with parallel evolution events, that is, the same virus mutation emerges in two different human hosts. FIGURE 6 | Haplotype network analysis using genome-wide single-nucleotide variations of VOC Delta in the world. Thiel V, Janke C, Guggemos W, Seilmaier M, phylogenetic network analysis of SARS-CoV-2 genomes. Several issues concerning the origin, time of introduction to humans, evolutionary patterns, and underlying force driving the SARS-CoV-2 outbreak remain unclear. However, in South America, this variant’s arrival and genomic diversity are scarcely The colors represent Most changes have little to no impact on the virus properties. Genetic recombination is a major evolutionary mechanism among RNA viruses, and it is common in coronaviruses, including those infecting humans. We all get that the closest known ancestor to SARS-CoV-2 is that bat virus. A few SARS-CoV-2 recombinants have been reported to date whose genome harbored combinations of mutations from different mutants or variants, but only a single patient’s sample was analyzed, and the virus was not isolated. Phylogenetic analysis of 48 SARS-Cov-2 isolates from Western Serbia (c) in comparison to Wuhan complete genome sequence (NCBI accession number NC_045512.2) and 31 most similar SARS-Cov-2 sequences obtained from other world regions through BLAST search. Looking at the phylogenetic tree, we see that a bat coronavirus is the closest relative to SARS-CoV-2, sharing around 96% of their genomes, says Lauring. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronavirus. Network, 26 Feb. 2020. First isolated in China in early 2020, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Phylogenetic network analysis of SARS-CoV-2 genomes. A recent phylogenetic network analysis of 160 SARS-Cov-2 genomes identified three central variants based on amino acid changes. This apparent convergent evolution was suggestive of natural selection and an adaptive sars-cov-2 is postulated to have originated from zoonotic transfer of a pangolin betacoronavirus based on a phylogenetic analysis of coronavirus sequences, due to a common insertion of 12 nucleotides within the receptor binding domain of the s protein region that optimizes binding to the human ace2 receptor, although the most similar The D614G mutation in the spike glycoprotein of SARS-CoV-2 was first detected at a significant level in early March 2020 and spread to global dominance over the next month. 2020;117(17):92413. Forster, L. Forster, C. Renfrew and M. Forster , Phylogenetic network analysis of SARS-CoV-2 genomes, Proc. 3 Menachery et al. on SARS-CoV-2 S protein mutations based on correlation network analysis Yoshiyuki Ogata* and Ruri Kitayama Abstract Background: Over a million genomes and mutational analyses of SARS-CoV-2 are available in public databases, which reveal the phylogenetic tree of the virus. Forster P, Forster L, Renfrew C, Forster M: Phylogenetic network analysis of SARS-CoV-2 genomes. The analyses revealed Europe and South-East Proc Natl Acad Sci U S A. J Mol Biol. Sci. 160 previously published viral genetic sequences from all over the world. But it is still a thousand nucleotide differences away from the human SARS-CoV-2 causing a pandemic at the moment. The most parsimonious tree with length = 42 663 is shown. Phylogenetic analysis of SARS-CoV-2 genomes in Turkey. Rep. 3 (2013) 1033. (A) The network analysis (integer neighbourhood joining network at reticulation tolerance value of 0.5, popART) of SARS-CoV-2 sequences from this study showing distinct clades with their geographical locations. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central variants distinguished by amino acid changes, which we have named A, B, and C, with A being the ancestral type according to the bat outgroup coronaviru Abstract. First, the ISM of a sequence preserves important nucleotide positions that can help to resolve different SARS-CoV-2 subtypes. A database for retrieving information on SARS-CoV-2 S protein mutations based on correlation network analysis Author: Yoshiyuki Ogata, Ruri Kitayama Source: BMC genomic data 2022 v.23 no.1 pp. The alignment of complete SARS-CoV- 2 genomes revealed that the new coronavirus had under- Since the genetic code of coronaviruses consists of RNA, a gone several mutations (Online Resource 2). Several issues concerning the origin, time of introduction to humans, evolutionary patterns, and underlying force driving the SARS-CoV-2 outbreak remain unclear. In a phylogenetic network analysis of 160 complete human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, we find three central Here is an example of EzCOVID19 analysis outputs. A phylogenetic network analysis of 160 early coronavirus genomes sampled from December 2019 to February 2020 showed that the virus type most closely related to the bat coronavirus was most abundant in Guangdong, China, and designated type "A". The article proposed a history of the pandemic and the existence of three subtypes affecting different populations. By analyzing large numbers of viral genomes together (blue lines), one can pinpoint sequences that are present only once in a given genome but also occur consistently SARS-CoV-2 has caused a severe, ongoing outbreak of COVID-19 in Massachusetts with 111,070 confirmed cases and 8,433 deaths as of August 1, 2020. Background SARS-CoV-2 began spreading in December 2019 and has since become a pandemic that has impacted many aspects of human society. Genomic surveillance of SARS-CoV-2 is one of the tools that provide genomic information on circulating variants. Infection, Genetics, Evolution. Phylogenetic trees comparing whole genomes of coronavirus species MERS-CoV, SARS-CoV, and NeoCoV, and trees A-D were built using different methods. Measuring about 30,000 RNA letters, SARS-CoV-2s genome is unusually long for an RNA virus. Our results demonstrate the following key features of ISM-based subtyping. The SARS-CoV-2 virus strains has geographical diversity associated with diverse severity, mortality rate, and response to treatment that were characterized using phylogenetic network analysis of SARS-CoV-2 genomes. 2 Ge et al. Although these data have enabled scientists to closely track the Report 5: Phylogenetic analysis of SARS-CoV-2 Erik Volz1, Marc Baguelin, Sangeeta Bhatia, Adhiratha Boonyasiri, Anne Cori, Zulma Cucunub, Gina Cuomo- Sampling of virus genomes was ad-hoc with all early samples originating from Wuhan, China, and a majority of recent samples originating from travellers outside of mainland China. Therefore, we performed phylogenetic analysis of S protein of the human pathogenic coronavirus Forster M. Phylogenetic network analysis of SARS-CoV-2 genomes. Early phylogenetic analysis of 160 SARS-CoV-2 genomes revealed three major variants named A, B, and C, with A being the ancestral type originated from the bat coronavirus from Wuhan (GenBank accession MG772933).